Detection of phlD gene in some fluorescent pseudomonads isolated from Iran and its relative with antifungal activities.
Identifieur interne : 001E96 ( Main/Exploration ); précédent : 001E95; suivant : 001E97Detection of phlD gene in some fluorescent pseudomonads isolated from Iran and its relative with antifungal activities.
Auteurs : Hamideh Afsharmanesh [Iran] ; Masoud Ahmadzadeh ; Abbas Sharifi-Tehrani ; Mohammad Javan-Nikkhah ; Keyvan GhazanfariSource :
- Communications in agricultural and applied biological sciences [ 1379-1176 ] ; 2007.
Descripteurs français
- KwdFr :
- Amplification de gène (MeSH), Antibactériens (biosynthèse), Antibiose (MeSH), Champignons (croissance et développement), Dépistage de masse (MeSH), Gènes fongiques (MeSH), Lutte biologique contre les nuisibles (méthodes), Numération de colonies microbiennes (MeSH), Phloroglucinol (analogues et dérivés), Phloroglucinol (métabolisme), Pseudomonas fluorescens (génétique), Pseudomonas fluorescens (métabolisme), Pseudomonas fluorescens (physiologie), Tests de sensibilité microbienne (MeSH), Variation génétique (MeSH).
- MESH :
- analogues et dérivés : Phloroglucinol.
- biosynthèse : Antibactériens.
- croissance et développement : Champignons.
- génétique : Pseudomonas fluorescens.
- métabolisme : Phloroglucinol, Pseudomonas fluorescens.
- méthodes : Lutte biologique contre les nuisibles.
- physiologie : Pseudomonas fluorescens.
- Amplification de gène, Antibiose, Dépistage de masse, Gènes fongiques, Numération de colonies microbiennes, Tests de sensibilité microbienne, Variation génétique.
English descriptors
- KwdEn :
- Anti-Bacterial Agents (biosynthesis), Antibiosis (MeSH), Colony Count, Microbial (MeSH), Fungi (growth & development), Gene Amplification (MeSH), Genes, Fungal (MeSH), Genetic Variation (MeSH), Mass Screening (MeSH), Microbial Sensitivity Tests (MeSH), Pest Control, Biological (methods), Phloroglucinol (analogs & derivatives), Phloroglucinol (metabolism), Pseudomonas fluorescens (genetics), Pseudomonas fluorescens (metabolism), Pseudomonas fluorescens (physiology).
- MESH :
- chemical , analogs & derivatives : Phloroglucinol.
- chemical , biosynthesis : Anti-Bacterial Agents.
- genetics : Pseudomonas fluorescens.
- growth & development : Fungi.
- chemical , metabolism : Phloroglucinol, Pseudomonas fluorescens.
- methods : Pest Control, Biological.
- physiology : Pseudomonas fluorescens.
- Antibiosis, Colony Count, Microbial, Gene Amplification, Genes, Fungal, Genetic Variation, Mass Screening, Microbial Sensitivity Tests.
Abstract
Fluorescent pseudomonads that produce antibiotic 2,4-diacetylphloroglocinol (2,4-DAPG) are important group of PGRP that inhibit a broad spectrum of plant pathogenic fungi. Studying on genetic diversity of 2,4-diacetylphloroglucinol-producing fluorescent pseudomonads has been shown with special importance. The first step to investigate the genetic diversity of these bacteria is detecting of the genes required for the biosynthesis of this antibiotic. The objectives of the current study were detection of phlD gene within fluorescent pseudomonads by a PCR-based assay, and comparison of phenotypic and genotypic characteristics of fluorescent pseudomonads with proven biocontrol potential against some soil-borne phytopathogenic fungi. We used a collection of 47 fluorescent Pseudomonas spp. some with known biological control activity against Macrophomina phaseolina, Rhizoctonia solani, Phytophthora nicotianae var. parasitica, Pythium sp. and Fusarium sp. in vitro and the potential to produce known secondary metabolites such as, siderophore, HCN and protease. The results indicated that 66, 40.42, 63.82,48.94 and 27.65% of strains revealed antagonistic activity against R. solani, M. phaseolina, Pythium sp., P. nicotianae and Fusarium sp., respectively. Rhizoctonia solani recognized as the most vulnerable fungus. Among 47 strains, 76.59, 97.87 and 17% of strains produced protease, siderophore and HCN, respectively. We could detect phlD gene in strains P-5, P-32, P-47. Strain CHA0 was used as positive control for the detection this gene. Overall, there was no obvious link between the existence of phlD gene and inhibition of fungal growth or production of the antifungal metabolites in vitro. But in some strains such as CHA0 and P-5, we saw a link between the existence of phlD and antifungal activities. Studying on detection and diversity of phlD provides a fundamental knowledge for developing a rapid genetic screening system to identify a potential biocontrol strains.
PubMed: 18396832
Affiliations:
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Ahmadzadeh</name></author><author><name sortKey="Sharifi Tehrani, Abbas" sort="Sharifi Tehrani, Abbas" uniqKey="Sharifi Tehrani A" first="Abbas" last="Sharifi-Tehrani">Abbas Sharifi-Tehrani</name></author><author><name sortKey="Javan Nikkhah, Mohammad" sort="Javan Nikkhah, Mohammad" uniqKey="Javan Nikkhah M" first="Mohammad" last="Javan-Nikkhah">Mohammad Javan-Nikkhah</name></author><author><name sortKey="Ghazanfari, Keyvan" sort="Ghazanfari, Keyvan" uniqKey="Ghazanfari K" first="Keyvan" last="Ghazanfari">Keyvan Ghazanfari</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:18396832</idno><idno type="pmid">18396832</idno><idno type="wicri:Area/Main/Corpus">001E17</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001E17</idno><idno type="wicri:Area/Main/Curation">001E17</idno><idno type="wicri:explorRef" wicri:stream="Main" 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sort="Ahmadzadeh, Masoud" uniqKey="Ahmadzadeh M" first="Masoud" last="Ahmadzadeh">Masoud Ahmadzadeh</name></author><author><name sortKey="Sharifi Tehrani, Abbas" sort="Sharifi Tehrani, Abbas" uniqKey="Sharifi Tehrani A" first="Abbas" last="Sharifi-Tehrani">Abbas Sharifi-Tehrani</name></author><author><name sortKey="Javan Nikkhah, Mohammad" sort="Javan Nikkhah, Mohammad" uniqKey="Javan Nikkhah M" first="Mohammad" last="Javan-Nikkhah">Mohammad Javan-Nikkhah</name></author><author><name sortKey="Ghazanfari, Keyvan" sort="Ghazanfari, Keyvan" uniqKey="Ghazanfari K" first="Keyvan" last="Ghazanfari">Keyvan Ghazanfari</name></author></analytic><series><title level="j">Communications in agricultural and applied biological sciences</title><idno type="ISSN">1379-1176</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anti-Bacterial Agents (biosynthesis)</term><term>Antibiosis (MeSH)</term><term>Colony Count, Microbial (MeSH)</term><term>Fungi (growth & development)</term><term>Gene Amplification (MeSH)</term><term>Genes, Fungal (MeSH)</term><term>Genetic Variation (MeSH)</term><term>Mass Screening (MeSH)</term><term>Microbial Sensitivity Tests (MeSH)</term><term>Pest Control, Biological (methods)</term><term>Phloroglucinol (analogs & derivatives)</term><term>Phloroglucinol (metabolism)</term><term>Pseudomonas fluorescens (genetics)</term><term>Pseudomonas fluorescens (metabolism)</term><term>Pseudomonas fluorescens (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amplification de gène (MeSH)</term><term>Antibactériens (biosynthèse)</term><term>Antibiose (MeSH)</term><term>Champignons (croissance et développement)</term><term>Dépistage de masse (MeSH)</term><term>Gènes fongiques (MeSH)</term><term>Lutte biologique contre les nuisibles (méthodes)</term><term>Numération de colonies microbiennes (MeSH)</term><term>Phloroglucinol (analogues et dérivés)</term><term>Phloroglucinol (métabolisme)</term><term>Pseudomonas fluorescens (génétique)</term><term>Pseudomonas fluorescens (métabolisme)</term><term>Pseudomonas fluorescens (physiologie)</term><term>Tests de sensibilité microbienne (MeSH)</term><term>Variation génétique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Phloroglucinol</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Anti-Bacterial Agents</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Phloroglucinol</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Antibactériens</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Champignons</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Pseudomonas fluorescens</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Pseudomonas fluorescens</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Phloroglucinol</term><term>Pseudomonas fluorescens</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Pest Control, Biological</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Phloroglucinol</term><term>Pseudomonas fluorescens</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Lutte biologique contre les nuisibles</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Pseudomonas fluorescens</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Pseudomonas fluorescens</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Antibiosis</term><term>Colony Count, Microbial</term><term>Gene Amplification</term><term>Genes, Fungal</term><term>Genetic Variation</term><term>Mass Screening</term><term>Microbial Sensitivity Tests</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amplification de gène</term><term>Antibiose</term><term>Dépistage de masse</term><term>Gènes fongiques</term><term>Numération de colonies microbiennes</term><term>Tests de sensibilité microbienne</term><term>Variation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Fluorescent pseudomonads that produce antibiotic 2,4-diacetylphloroglocinol (2,4-DAPG) are important group of PGRP that inhibit a broad spectrum of plant pathogenic fungi. Studying on genetic diversity of 2,4-diacetylphloroglucinol-producing fluorescent pseudomonads has been shown with special importance. The first step to investigate the genetic diversity of these bacteria is detecting of the genes required for the biosynthesis of this antibiotic. The objectives of the current study were detection of phlD gene within fluorescent pseudomonads by a PCR-based assay, and comparison of phenotypic and genotypic characteristics of fluorescent pseudomonads with proven biocontrol potential against some soil-borne phytopathogenic fungi. We used a collection of 47 fluorescent Pseudomonas spp. some with known biological control activity against Macrophomina phaseolina, Rhizoctonia solani, Phytophthora nicotianae var. parasitica, Pythium sp. and Fusarium sp. in vitro and the potential to produce known secondary metabolites such as, siderophore, HCN and protease. The results indicated that 66, 40.42, 63.82,48.94 and 27.65% of strains revealed antagonistic activity against R. solani, M. phaseolina, Pythium sp., P. nicotianae and Fusarium sp., respectively. Rhizoctonia solani recognized as the most vulnerable fungus. Among 47 strains, 76.59, 97.87 and 17% of strains produced protease, siderophore and HCN, respectively. We could detect phlD gene in strains P-5, P-32, P-47. Strain CHA0 was used as positive control for the detection this gene. Overall, there was no obvious link between the existence of phlD gene and inhibition of fungal growth or production of the antifungal metabolites in vitro. But in some strains such as CHA0 and P-5, we saw a link between the existence of phlD and antifungal activities. Studying on detection and diversity of phlD provides a fundamental knowledge for developing a rapid genetic screening system to identify a potential biocontrol strains.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18396832</PMID><DateCompleted><Year>2008</Year><Month>06</Month><Day>02</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1379-1176</ISSN><JournalIssue CitedMedium="Print"><Volume>72</Volume><Issue>4</Issue><PubDate><Year>2007</Year></PubDate></JournalIssue><Title>Communications in agricultural and applied biological sciences</Title><ISOAbbreviation>Commun Agric Appl Biol Sci</ISOAbbreviation></Journal><ArticleTitle>Detection of phlD gene in some fluorescent pseudomonads isolated from Iran and its relative with antifungal activities.</ArticleTitle><Pagination><MedlinePgn>941-50</MedlinePgn></Pagination><Abstract><AbstractText>Fluorescent pseudomonads that produce antibiotic 2,4-diacetylphloroglocinol (2,4-DAPG) are important group of PGRP that inhibit a broad spectrum of plant pathogenic fungi. Studying on genetic diversity of 2,4-diacetylphloroglucinol-producing fluorescent pseudomonads has been shown with special importance. The first step to investigate the genetic diversity of these bacteria is detecting of the genes required for the biosynthesis of this antibiotic. The objectives of the current study were detection of phlD gene within fluorescent pseudomonads by a PCR-based assay, and comparison of phenotypic and genotypic characteristics of fluorescent pseudomonads with proven biocontrol potential against some soil-borne phytopathogenic fungi. We used a collection of 47 fluorescent Pseudomonas spp. some with known biological control activity against Macrophomina phaseolina, Rhizoctonia solani, Phytophthora nicotianae var. parasitica, Pythium sp. and Fusarium sp. in vitro and the potential to produce known secondary metabolites such as, siderophore, HCN and protease. The results indicated that 66, 40.42, 63.82,48.94 and 27.65% of strains revealed antagonistic activity against R. solani, M. phaseolina, Pythium sp., P. nicotianae and Fusarium sp., respectively. Rhizoctonia solani recognized as the most vulnerable fungus. Among 47 strains, 76.59, 97.87 and 17% of strains produced protease, siderophore and HCN, respectively. We could detect phlD gene in strains P-5, P-32, P-47. Strain CHA0 was used as positive control for the detection this gene. Overall, there was no obvious link between the existence of phlD gene and inhibition of fungal growth or production of the antifungal metabolites in vitro. But in some strains such as CHA0 and P-5, we saw a link between the existence of phlD and antifungal activities. Studying on detection and diversity of phlD provides a fundamental knowledge for developing a rapid genetic screening system to identify a potential biocontrol strains.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Afsharmanesh</LastName><ForeName>Hamideh</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Faculty of Horticultural Sciences and Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. h_afsharmanesh@yahoo.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ahmadzadeh</LastName><ForeName>Masoud</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Sharifi-Tehrani</LastName><ForeName>Abbas</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Javan-Nikkhah</LastName><ForeName>Mohammad</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Ghazanfari</LastName><ForeName>Keyvan</ForeName><Initials>K</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Belgium</Country><MedlineTA>Commun Agric Appl Biol Sci</MedlineTA><NlmUniqueID>101200320</NlmUniqueID><ISSNLinking>1379-1176</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000900">Anti-Bacterial Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>8XV4YYO3WN</RegistryNumber><NameOfSubstance UI="C059817">2,4-diacetylphloroglucinol</NameOfSubstance></Chemical><Chemical><RegistryNumber>DHD7FFG6YS</RegistryNumber><NameOfSubstance UI="D010696">Phloroglucinol</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000900" MajorTopicYN="N">Anti-Bacterial Agents</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000898" MajorTopicYN="N">Antibiosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015169" MajorTopicYN="N">Colony Count, Microbial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005784" MajorTopicYN="N">Gene Amplification</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005800" MajorTopicYN="N">Genes, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014644" MajorTopicYN="Y">Genetic Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008403" MajorTopicYN="N">Mass Screening</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008826" MajorTopicYN="N">Microbial Sensitivity Tests</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010572" MajorTopicYN="N">Pest Control, Biological</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010696" MajorTopicYN="N">Phloroglucinol</DescriptorName><QualifierName UI="Q000031" MajorTopicYN="N">analogs & derivatives</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011551" MajorTopicYN="N">Pseudomonas fluorescens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>4</Month><Day>10</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>6</Month><Day>3</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>4</Month><Day>10</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18396832</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Iran</li></country></list><tree><noCountry><name sortKey="Ahmadzadeh, Masoud" sort="Ahmadzadeh, Masoud" uniqKey="Ahmadzadeh M" first="Masoud" last="Ahmadzadeh">Masoud Ahmadzadeh</name><name sortKey="Ghazanfari, Keyvan" sort="Ghazanfari, Keyvan" uniqKey="Ghazanfari K" first="Keyvan" last="Ghazanfari">Keyvan Ghazanfari</name><name sortKey="Javan Nikkhah, Mohammad" sort="Javan Nikkhah, Mohammad" uniqKey="Javan Nikkhah M" first="Mohammad" last="Javan-Nikkhah">Mohammad Javan-Nikkhah</name><name sortKey="Sharifi Tehrani, Abbas" sort="Sharifi Tehrani, Abbas" uniqKey="Sharifi Tehrani A" first="Abbas" last="Sharifi-Tehrani">Abbas Sharifi-Tehrani</name></noCountry><country name="Iran"><noRegion><name sortKey="Afsharmanesh, Hamideh" sort="Afsharmanesh, Hamideh" uniqKey="Afsharmanesh H" first="Hamideh" last="Afsharmanesh">Hamideh Afsharmanesh</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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